Thermo Fisher Mass Spectrometer Q EXACTIVE MD As-is, CLEARANCE!

Thermo Fisher Scientific Mass Spectrometer

MODEL:Q EXACTIVE MD

Transforming discovery quantitation

The Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer features an ultra-high-field Orbitrap analyzer which doubles its speed and resolution – more sample runs and amazing data quality. Built on the proven performance of the Q Exactive Plus platform the Q Exactive HF mass spectrometer utilizes the same Active Beam Guide technology and Advanced Quadrupole design for very stable system operation and exceptional analytical performance. The Q Exactive HF mass spectrometer is designed for your toughest discovery and quantitation challenges where the combination of speed and highest performance are essential for the highest quality scientific results.

• Ultra-High-Field Orbitrap (HF) mass analyzer for faster scan speed and higher resolution
• Advanced Quadrupole Technology (AQT) improves precursor selection and transmission
for more accurate quantitation of low abundant analytes in complex matrices
• Sophisticated Data-Independent Acquisition (DIA) and Parallel Reaction Monitoring (PRM)
deliver reproducible quantitation with complete qualitative confidence
• The Advanced Active Beam Guide (AABG) reduces noise and improves instrument robustness
• Optional intact Protein Mode provides superior trapping of large molecules for improved
analysis of intact proteins

Analyze complex mixtures faster than ever!

Analyze complex mixtures faster than ever!
The Q Exactive HF mass spectrometer, with an ultra-high-field Thermo Scientific™ Orbitrap™ detector, provides new
levels of speed and resolution for every scan while maintaining high spectral quality for sensitive detection and
confident quantitation of peptides, lipids, metabolites, contaminants and other important compound classes.

Quantify with the highest confidence

With its unique spectrum-multiplexing capabilities and faster scan speed, the Q Exactive HF mass
spectrometer provides the speed necessary for precise quantitation even for narrow ultra-high-performance
liquid chromatography (UHPLC). Industry leading high resolution accurate mass (HR/AM) MS provides you
with a more complete quantitative picture.

A digital data archive – New levels of DIA capability

Targeted quantitative methods deliver the highest sensitivity, best precision, and lowest detection limits, but require a priori
knowledge of the precursor ions (target molecules) and significant time and effort for SRM or PRM method development
and optimization.
In a true discovery quantitation environment, the targets may not be known and relative quantitation may be sufficient. In such
cases, data independent acquisition, is a viable quantitative approach. The Q Exactive HF MS enables several methods for DIA
data acquisition, including BASIC-DIA, pSMART-DIA, and MSx-DIA. These DIA methods fragment all ions present in the sample
to create a high-quality, permanent digital data archive of a sample that can be reanalyzed to look for new targets.

Superior intact protein characterization

The analysis of intact proteins presents a different set of challenges in which maximum resolution of thespectral features can help to distinguish major protein isoforms from lower abundant species. Particularly in biopharmaceutical applications, the analysis of heterogeneous protein mixtures requires the highest
resolving capabilities combined with the high mass accuracy provided only with Orbitrap technology.The Q Exactive HF MS, with the optional Intact Protein Mode, enables high resolution analysis of intact proteins, allowing detailed characterization of larger, more highly charged protein species such as intact antibodies, as well as the individual heavy and light chains. With the additional resolving power of the
Q Exactive HF MS, proteins as large as 50 kDa can be isotopically resolved. This improved resolution allows routine, detailed characterization of proteins and biopharmaceuticals.

Increased productivity and confidence for lipidomics

The analysis of lipids poses a unique analytical challenge
in research, as the closely related molecular structure of
many lipid species produce multiple isobaric species within
a relatively narrow mass range. This creates the risk of
ambiguous lipid structure assignments. The high resolving
power of the Q Exactive HF system greatly reduces this
risk compared to instrumentation with lower resolving power.
The Q Exactive HF MS is ideal for both infusion and LC-based
workflows. Fast acquisition speed allows the system to keep
pace with demanding lipid separations from complex samples.